The performance of UniSafe+ was tested by carrying out standard suspension tests. Such tests follow a recognised approach to disinfectant validation which is the CEN (European Committees for normalisation) 216 work programme and provides a systematic approach to the testing of disinfectants.

From this a number of BS EN methods have been derived for which there are clearly defined pass criteria. In particular the BS EN methods are standard suspension tests that are phase2/step1 tests.

This means that the test is appropriate to the intended use of the biocidal product.

Suspension tests are set up with 1 part micro-organism suspension, the count of micro-organisms to be within defined limits, 1 part interfering substance and 8 parts biocidal agent.

Contact between the biocide and micro-organism/interfering substance mixture is maintained for a defined time at a defined temperature (20degrees+/-1degreeC) - the biocide is then neutralised appropriately.

Controls ensure correct neutralisation of the product has occurred and internally validate the test. Tests require that the biocidal agent reduces the microbiological viability by 5 1og10 (bacteria) or 4 log10 (Fungi) or 3 log10 (Spores) or 4 log10 (Viruses).